There are greater than 70 species of flavivirus, and lots of trigger illnesses in people and animals, together with dengue, Zika, and yellow fever viruses. A novel flavivirus identification take a look at that’s each quick and delicate has been validated in Brazil by Mariana Sequetin Cunha and collaborators on the Adolfo Lutz Institute, a number one epidemiological surveillance laboratory that studies to the São Paulo state authorities.
An article on the subject has been revealed in Archives of Virology.
The analysis was supported by São Paulo Research Foundation—FAPESP by way of a Thematic Project, for which the principal investigator (PI) was Maurício Lacerda Nogueira, and a Regular Research Grant for which the PI was Paulo Cesar Maiorka.
“We set out to improve the monitoring of flaviviruses in Brazil by means of a reliable method. To this end, we used the RT-qPCR assay technique,” Sequetin stated. RT-qPCR stands for reverse transcription quantitative polymerase chain response. The laboratory approach combines reverse transcription of RNA into DNA and amplification of particular DNA targets utilizing polymerase chain response. It is taken into account the gold normal for speedy identification of viruses and is advisable by the World Health Organization for diagnosing infection by SARS-CoV-2.
“Until recently, the main method used in Brazil to identify flaviviruses required inoculating the brains of newborn mice with suspected material sampled from human patients or animals,” Sequetin stated. “When I joined the Adolfo Lutz Institute as a researcher in 2012, I decided to establish an alternative method that would not require the mice but would submit the patient’s blood, serum or viscera sample directly to RT-qPCR.”
The key query was whether or not RT-qPCR could be delicate sufficient to detect small quantities of viruses within the samples analyzed. Sequetin recalled that the Adolfo Lutz Institute maintained a lot of deep-frozen mice that had been inoculated within the 1990s and saved at -80 °C. “I extracted genetic material from their brains and sought the threshold for detection of different flaviviruses by preparing increasingly dilute solutions,” she stated.
The protocol established was proven to be extremely delicate and particular. It can be utilized to detect the completely different flaviviruses that happen in Brazil and for viral monitoring in sentinel animals and vectors.
“We’re going to test it on new samples that we’re receiving. I expect to find flaviviruses not described in the literature, especially in mosquitos,” Sequetin stated.