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Advanced Biofuels USA

Enzyme Cocktail Developed in Brazil Powers Production of Second-Generation Ethanol

Publicado em 19 agosto 2020

Brazilian researchers used genetic engineering to develop a low-cost platform for the production of enzymes that break down sugarcane trash and bagasse for conversion into biofuel. The novel molecules have many potential industrial applications.

Researchers at the Brazilian Center for Research in Energy and Materials (CNPEM) have genetically engineered a fungus to produce a cocktail of enzymes that break down the carbohydrates in biomass, such as sugarcane trash (tops and leaves) and bagasse, into fermentable sugar for industrially efficient conversion into biofuel.

The development of low-cost enzyme cocktails is one of the main challenges in producing second-generation ethanol.

Second-generation biofuels are manufactured from various kinds of nonfood biomass, including agricultural residues, wood chips and waste cooking oil. The CNPEM research group’s process paves the way for optimized use of sugarcane residues to produce biofuels.

The fungus Trichoderma reesei is one of the most prolific producers of plant cell wall-degrading enzymes and is widely used in the biotechnology industry. To enhance its productivity as a biofactory for the enzyme cocktail in question, the researchers introduced six genetic modifications into RUT-C30, a publicly available strain of the fungus. They patented the process and reported it in an article published in the journal Biotechnology for Biofuels.  READ MORE

Rational engineering of the Trichoderma reesei RUT-C30 strain into an industrially relevant platform for cellulase production (Biotechnology for Biofuels)

by Maria Fernanda Ziegler (Agência FAPESP/EurekAlert!)